Description
Principle of the Assay::The Mouse CD32 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD32 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD32 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD32 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD32 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD32 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body